Table 1.
The sequences of primers used for construction of TRIM22 (GenBank: NM_006074), and N (GenBank: KY964305.1) protein mutants
| Name | Primer sequence (5′-3′) |
|---|---|
| TRIM22-F | TTTAAGCTTATGGATTTCTCAGTAAAGGTAGACATA |
| TRIM22-R | TTTCTCGAGGGAGCTCGGTGGGCACACAGTCATGGG |
| ΔRING-F | TTTAAGCTTCAGACCAGATTCCAGCCTGGGAACCTC |
| SPRY-F | TTTAAGCTTAAGAAGCCAAAATCTGTTTCCAAGAAA |
| ΔSPRY-R | TTTCTCGAGGTACTGGACATCTGTCAGCTCTTTAAG |
| ΔNLS-F | GAGTGAAAGCTGGACATTGAGTGTATTCCGAGTACCAG |
| ΔNLS-R | CTGGTACTCGGAATACACTCAATGTCCAGCTTTCACTC |
| ORF7-F | TTTGAATTCATGCCAAATAACAACGGCAAGCAGC |
| ORF7-R | TTTAGATCTTCATGTTGGGGGTGATGCTGTGGCG |
| K10-4A-F1 | AGCAAGCGGCAGCGGCGGGGAATGGCCAGCCAGTCGA |
| K10-4A-F2 | ATAACAACGGCAAGCAGCAAGCGGCAGCGGCGGGGAATG |
| C23A-F1 | AGCCAGTCGATCAGCTGGCCCAAATGCTGGGTAAGATCA |
| C23A-F2 | AAAGAAAAAGAAGGGGAATGGCCAGCCAGTCGATCAGCTGG |
| C23A-F3 | ATAACAACGGCAAGCAGCAAAAGAAAAAGAAGGGGAA |
| Q32-4A-F | CATCGCCGCAGCAGCCGCGTCCAGAGGCAAGGGACCG |
| Q32-4A-R | CTCTGGACGCGGCTGCTGCGGCGATGATCTTACCCAG |
| K43-2A-F | ACCGGGGGCGGCAAATAGGAAGAAAAACCCG |
| K43-2A-R | CTTCCTATTTGCCGCCCCCGGTCCCTTGCCTCTG |
| P50-3A-F | CGGAGAAGGCCCATTTCGCTCTAGCGACTGAAGATGACG |
| P50-3A-R | GCGAAATGGGCCTTCTCCGCGTTTTTCTTCCTATTTTTC |
The restriction enzyme sites used for cloning are underlined in italics. Locations of mutations are underlined