Table 2.
Detection of PEDV and TGEV in 114 clinical samples with duplex nanoPCR and conventional PCR assays
| Province or city | No. of clinical samples | Viruses detected | No. of positive samples detected by duplex nanoPCR | No. of positive samples detected by conventional | Positive (%) with duplex nanoPCR | Positive (%) with conventional PCR |
|---|---|---|---|---|---|---|
| Hebei | 91 | PEDV | 47 | 45 | 51.6 | 49.5 |
| TGEV | 4 | 4 | 4.4 | 4.4 | ||
| PEDV+TGEV | 3 | 3 | 3.3 | 3.3 | ||
| Beijing | 5 | PEDV | 2 | 2 | 40 | 40 |
| TGEV | 0 | 0 | 0 | 0 | ||
| PEDV+TGEV | 0 | 0 | 0 | 0 | ||
| Zhejiang | 3 | PEDV | 2 | 2 | 66.7 | 66.7 |
| TGEV | 0 | 0 | 0 | 0 | ||
| PEDV+TGEV | 0 | 0 | 0 | 0 | ||
| Guangdong | 9 | PEDV | 3 | 3 | 33.3 | 33.3 |
| TGEV | 0 | 0 | 0 | 0 | ||
| PEDV+TGEV | 0 | 0 | 0 | 0 | ||
| Others | 6 | PEDV | 1 | 0 | 16.7 | 0 |
| TGEV | 0 | 0 | 0 | 0 | ||
| PEDV+TGEV | 0 | 0 | 0 | 0 | ||
| Total | 114 | PEDV | 55 | 52 | 48.2 | 45.6 |
| TGEV | 4 | 4 | 3.5 | 3.5 | ||
| PEDV+TGEV | 3 | 3 | 2.6 | 2.6 |