Fig. 1.

Schematic representation of PCRD nucleic acid detector design. At the conjugate pad, the carbon Biotin-conjugated antibodies bind to biotin on one end of the amplicons, and flows towards the test-lines, L1 and L2. L1 is lined with anti-DIG monoclonal antibodies to capture DIG/Biotin labeled amplicons for NDV assay. L2 is lined with anti-FAM monoclonal antibodies to capture FAM/Biotin labeled amplicons for IBV assay. The excess carbon Biotin-conjugated antibodies are captured by anti-mouse antibodies at the control line (C)