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. 2020 Mar 23;15(3):e0230586. doi: 10.1371/journal.pone.0230586

Fig 5. Quantitative analysis of the collecting duct morphological features and function in Vangl2 mutant mice.

Fig 5

(A) Longitudinal measurement of collecting duct cross-sectional area ratio in E17.5—P90 mice: ubiquitous mutants Vangl2Lp/Lp (n = 310), Vangl2Δ/Δ (n = 108), conditional mutant Vangl2Δ/CD (E17.5 n = 93, P1 n = 362, P7 n = 163, P30 n = 221, P90 n = 117), Cre(-);Vangl2Fl/Fl (E17.5 n = 93, P1 n = 324, P7 n = 194, P30 n = 245, P90 n = 128) and Cre(+);Vangl2+/+ (P1 n = 337, P7 n = 109, P30 n = 212, P90 n = 141) (two latter are controls). (B) Longitudinal measurement of collecting duct cross-sectional cell counts in the same structures as in (A). (C) The upper quartile area ratio in the collecting duct tubules in ubiquitous mutant Vangl2Δ/Δ (E17.5 n = 20), conditional Vangl2Δ/CD (E17.5 n = 27, P1 n = 78, P7 n = 41), mutants and Cre(-);Vangl2Fl/Fl (E17.5 n = 22, P1 n = 85, P7 n = 46) and Cre(+);Vangl2+/+ (P1 n = 83, P7 n = 25) at E17.5, P1 and P7. (D) The upper quartile cell counts in the collecting duct tubule structures used in (C). (E) Urine K+/Na2+ ratio in P30-9 month-old conditional Vangl2Δ/CD and control Cre(+) Vangl2+/+ and Cre(-);Vangl2Fl/Fl mice: minimum 3 specimens per time point per genotype were used. (F) Urine pH in P30–9 month-old conditional Vangl2Δ/CD and control Cre(+) Vangl2+/+/ Cre(-);Vangl2Fl/Fl mice: minimum 6 specimens per time point per genotype were used. The data represent mean ± standard error of mean in all graphs. p<0.05 (*), p<0.01 (**), p <0.001 (***).