Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | BL21 (DE3) | EMBL protein expression facility | NA | |
| Strain, strain background (Escherichia coli) | BL21 Rosetta 2 | Merck Millipore | Cat #71400–3 | |
| Recombinant DNA reagent | pETM-11 Fibrillarin (plasmid) | Lapinaite et al. (2013) | Nterminal His6 + TEV site | |
| Recombinant DNA reagent | pETM-11 Nop5 (plasmid) | Lapinaite et al. (2013) | Nterminal His6 + TEV site; L113K V223E mutant. Codon-optimised synthetic gene (GeneArt) | |
| Recombinant DNA reagent | pETM-11 Nop5 E65C (plasmid) | Lapinaite et al. (2013) | Mutation of pETM-11 Nop5 | |
| Recombinant DNA reagent | pETM-11 Nop5 E196C (plasmid) | Lapinaite et al. (2013) | Mutation of pETM-11 Nop5 | |
| Recombinant DNA reagent | pETM-11 Nop5 D247C (plasmid) | Lapinaite et al. (2013) | Mutation of pETM-11 Nop5 | |
| Recombinant DNA reagent | pETM-11 Nop5 S343C (plasmid) | Lapinaite et al. (2013) | Mutation of pETM-11 Nop5 | |
| Recombinant DNA reagent | pETM-11 L7Ae (plasmid) | Lapinaite et al. (2013) | Nterminal His6 + TEV site | |
| Recombinant DNA reagent | pETM-11 L7Ae Q45C (plasmid) | Lapinaite et al. (2013) | Mutation of pETM-11 L7Ae also carrying C68S mutation | |
| Recombinant DNA reagent | pETM-11 L7Ae E58C (plasmid) | Lapinaite et al. (2013) | Mutation of pETM-11 L7Ae also carrying C68S mutation | |
| Sequence-based reagent | st-sR26 | Graziadei et al. (2016) | In vitro transcribed RNA | |
| Sequence-based reagent | st-sR26-1 | This paper | In vitro transcribed RNA | Method section: RNA synthesis |
| Sequence-based reagent | st-sR26-1 substrate | This paper | In vitro transcribed RNA | Method section: RNA synthesis |
| Sequence-based reagent | st-sR26-1 A61C | This paper | In vitro transcribed RNA | Method section: RNA synthesis |
| Sequence-based reagent | st-sR26-1 A61U | This paper | In vitro transcribed RNA | Method section: RNA synthesis |
| Sequence-based reagent | st-sR26-2 | This paper | In vitro transcribed RNA | Method section: RNA synthesis |
| Sequence-based reagent | st-sR26-2 substrate | This paper | In vitro transcribed RNA | Method section: RNA synthesis |
| Sequence-based reagent | sR26 | Graziadei et al. (2016) | In vitro transcribed RNA | |
| Sequence-based reagent | ssR26 | Lapinaite et al. (2013) | In vitro transcribed RNA | |
| Commercial assay or kit | TLAM-ILVproS labelling | NMR-Bio | NA | |
| Chemical compound, drug | Iodoacetoamido-PROXYL | Sigma-Aldrich | Cat # 253421–25 MG | |
| Chemical compound, drug | (methyl-13C, 99%; 3,3-D2, 98%) α-ketobutyric acid | Cambridge Isotope Labs | CDLM-7318-PK | |
| Chemical compound, drug | (3-methyl-13C, 99%; 3,4,4,4-D4, 98%) α-ketoisovaleric acid | Cambridge Isotope Labs | CDLM-7317-PK | |
| Chemical compound, drug | [3–2 H2,4–2H, 5–13C, 5’−2 H3]-a-ketoiso-caproate | Lichtenecker et al. (2013) | ||
| Software, algorithm | CNS | This paper | Method section: Structure calculation and selection. Adaptation of protocol from Lapinaite et al. (2013) | |
| Software, algorithm | Python-based SAS-PRE scoring algorithm | This paper | ||
| Software, algorithm | ATSAS 2.7.5 | Petoukhov et al., 2012 | ||
| Software, algorithm | Python-based SAS-PRE scoring algorithm | This paper | Method section: Ensemble Scoring |