Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (E. coli) | TKL130 | (Lee et al., 2014) | MG1655 mrdA::PAmCherry-mrdA | |
| Strain, strain background (E. coli) | EW07 | This work | TKL130 ΔmreCD, pFB121 | Materials and methods, Strain construction |
| Strain, strain background (E. coli) | EW49 | This work | TKL130 ΔrodZ, pFB290 | Materials and methods, Strain construction |
| Strain, strain background (E. coli) | AV48 | This work | 186attB::Ptet-dcas9, mrdA::rPAmCherry-mrdA | Materials and methods, Strain construction |
| Strain, strain background (E. coli) | NO53 | (Ouzounov et al., 2016) | MG1655 mreB-msfGFPsw | |
| Strain, strain background (E. coli) | TU230(attLHC943) | (Rohs et al., 2018) | MG1655 mrdA::aph (Plac::msfgfp-mrdA) | |
| Strain, strain background (E. coli) | TU230(attLPR122) | (Rohs et al., 2018) | MG1655 mrdA::aph (Plac::msfgfp-pbpA(L61R)) | |
| Strain, strain background (E. coli) | S352 | This work | MG1655 ΔponA::aph | Materials and methods, Strain construction |
| Strain, strain background (E. coli) | FB83 | (Bendezú and de Boer, 2008) | MG1655, lacIZYA::frt, mreB-mCherrySW yhdE::frt | |
| Strain, strain background (E. coli) | AV07 | (Vigouroux et al., 2018) | MG1655 mrdA::mcherry-mrdA | |
| Strain, strain background (E. coli) | AV127 | This work | 186::dCas9, mrdA::sfgfp-mrdA | Materials and methods, Strain construction |
| Strain, strain background (E. coli) | FB60 (iFB273) | (Bendezú and de Boer, 2008) | MG1655 lacIZYA::frt, rodZ::aph, Plac::gfp-rodZ | |
| Strain, strain background (E. coli) | S504 | This work | FB83, rodZ::aph, Plac::gfp-rodZ | Materials and methods, Strain construction |
| Strain, strain background (E. coli) | S505 | This work | AV07, rodZ::aph, Plac::gfp-rodZ | Materials and methods, Strain construction |
| Chemical compound, drug | Mecillinam | Sigma-Aldrich, #33447 | ||
| Chemical compound, drug | A22 | Cayman Chemical #15870 | ||
| Software, algorithm | Trackmate | (Tinevez et al., 2017) | ||
| Software, algorithm | Morphometrics | (Ursell et al., 2017) | ||
| Software, algorithm | Spot-On | (Hansen et al., 2018) |