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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Nov 26;198:105552. doi: 10.1016/j.jsbmb.2019.105552

Figure 1. Generation of a CAMP transgenic mouse and expression in different tissues.

Figure 1.

(A) This schematic generated from the UCSC Genome Browser December 2013 (GRCh38/hg38) Assembly shows that the human 6.02 kb Kpn1-EcoR1 genomic DNA fragment contains the CAMP gene located on chromosome 3 (base pairs 48,223,347 −48,225,491). It also shows the conserved 5’- and 3’-regions that possess potentially important transcriptional regulatory elements and the VDRE located in a primate-specific AluSx SINE conserved in only apes (chimp, gorilla, orangutan and gibbon), Old World primates (rhesus macaque, baboon and green monkey) and New World primates (Ma’s night monkey and marmoset), but not in prosimians (tarsier, black lemur and bushbaby) or other mammals (mouse, rat, dog and opossum). (B) The human CAMP mRNA is expressed in tissues of Tg mice. Total RNA was isolated from tissues of Tg/WT mice and expression of CAMP mRNA was measured by qRT-PCR. The ratio of CAMP to the reference gene Ywhaz was log10 transformed and graphed as the mean (+S.D.) with the minimum and maximum (Box plots, n=4–6 mice; non-box plots, n=3 mice).