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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Nov 26;198:105552. doi: 10.1016/j.jsbmb.2019.105552

Figure 3. Human CAMP gene does not alter phagocytosis or superoxide production in murine phagocytes.

Figure 3.

(A) BM-derived macrophages from WT, Tg/KO, and KO genotypes were incubated with fluorescent pHrodo E. coli for 60 min and then analyzed by flow cytometry to measure the percentage of phagocytes. Data represent mean (+SD) of four experiments. (B) BM-derived macrophages from WT and Tg/WT mice were incubated with a luminol substrate and stimulated with PMA to induce superoxide as measured by relative light unit (RLU) emission over a time course. Each time point represents the mean (±SD) of three experiments. (C) BM neutrophils from WT and Tg/WT mice were incubated as described in panel B. Each time point represents the mean (±SD) of six biological replicates from two experiments for each genotype. No significant differences were observed between the genotypes.