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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Hepatology. 2020 Feb 14;71(6):2118–2134. doi: 10.1002/hep.30966

Figure 6. Beneficial effects of downregulation of miR-210 in cholestatic mice are largely dependent on MLL4.

Figure 6.

Mice were injected with empty lentivirus (Lenti-empty) or lenti-virus expressing miR-210 shRNA (Lenti-sh-miR-210), 2 weeks later mice were injected with empty lentivirus (Lenti-empty) or lentivirus expressing shRNA for Mll4 (Lenti-sh-Mll4), and 2 weeks later the mice were fed CA-chow for 1 week. (A) Experimental approach (left) and legends showing viral injection groups (right). (B) Levels of miR-210 in liver and plasma (left) and hepatic Mll4 mRNA (right) (C) Levels of mRNAs of the indicated genes. (D) Gallbladder volume (n=5), levels of BAs in the liver, intestine, and plasma and ALT in the plasma. (E) Liver sections stained with H&E (upper panels) or by IHC with F4/80 antibody (lower panels). Statistical significance was determined by two-way ANOVA (B, C, E), SD (n=4–5), * p < 0.05, ** p < 0.01, NS, not significant.