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Clinical characterization and antibody titers in MuSK-EAMG, induced in FVB/N mice. FVB/N female mice were immunized twice with 20–40 μg (as indicated) of recombinant MuSK in CFA, or with CFA alone, as a control (n = 6). Mice were followed up for clinical score (A) and weight loss changes (B). Anti-MuSK antibody titer was tested 4 weeks following immunization, by ELISA (C), and correlation with disease severity was tested (D). P < 0.001 in (A,B). Analyzed by the two-way ANOVA test.
