Table 5.
Expression of antifungal peptides in fungi and plants.
| Type of AFP | Name | Source | Host | Expression vector | Fusion partner | Antifungal spectrum | Yield | Reference |
|---|---|---|---|---|---|---|---|---|
| FUNGI | ||||||||
| Aureobasidins | Aureobasidin A (BP-1938) | Aureobasidium pullulans | A. pullulans | pCR2.1 TOPO | None | Candida spp. | Unknown | Slightom et al., 2009 |
| Cysteine rich | Neosartorya fischeri antifungal protein 2 (NFAP2) | N. fischeri |
Penicillium chrysogenum Q176 GRAS-FDA |
pSK275nfap | None | Candida spp. | 15 mg/L | Tóth et al., 2018 |
| Cysteine rich | AfpB | P. digitatum CECT 20796 (PHI26) |
P. digitatum
P. pastoris X-33 |
pBHt2 pPICZαA |
None |
Saccharomyces cerevisiae, P. italicum, P. expansum, Botrytis cinerea, Magnaporthe oryzae, Fusarium oxysporum, P. digitatum |
12–20 mg protein/l (P. digitatum) 1.2–1.4 mg/l (P. pastoris) |
Garrigues et al., 2018 |
| PLANTS | ||||||||
| Lactoferrin-derived peptides | Lactoferrin+lactoferrampin chimera | Bovine milk | Nicotiana tabacum | pBI121 | His tag | C. tropicalis, C. krusei, C. albicans, C. glabrata, Aspergillus spp., Cryptococcus spp. | 4.8 μg/g fresh weight | Chahardoli et al., 2018 |
| Dermaseptin | Dermaseptin B1 | Skin glands of the South American hylid frog, Phyllomedusa bicolor | Nicotiana tabacum | pGSA1285 | Tandem repeat of Cladosporium fulvum Avr4 effector protein CBD | Agrobacterium tumefaciens (PTCC 1654), Pectobacterium carotovorum (PTCC 1675), Pseudomonas aeruginosa (PTCC 1558), Xanthomonas campestris (PTCC 1473), and Ralstonia solanacearum (ATCC 11696) bacteria, Alternaria alternata (PTCC 5224) and Pythium spp. | Unknown | Shams et al., 2019 |