Figure 3.

Cellular immune response against DENV3 in mouse splenocytes. Splenocytes were isolated 1 week after the final immunization. (A–D) The antigen-experienced CD8⁺ T cell response was assayed by flow cytometry (n = 8). (A) Representative images of CD44⁺ CD62L⁻ T cells (gated on CD3e⁺ CD8⁺ T cells). (B) Quantification of the frequency of CD44⁺ CD62L⁻ CD8⁺ T cells. The data are expressed as the mean percentage ± SD from three independent experiments. (C) Representative images of CD11a⁺ IFN-γ⁺ T cells (gated on CD3e⁺ CD8⁺ T cells). (D) Quantification of the frequency of CD11a⁺ IFN-γ⁺ CD8⁺ T cells. The data are expressed as the mean percentage ± SD from three independent experiments. (E,F) Splenocyte-secreted cytokines detected by ELISPOT assay (n = 8). The number of cytokine-positive cells is recorded as the mean spot-forming unit (SFU)/5 × 10⁵ splenocytes ± SD. ***p < 0.001.