Figure 6.

Short-term cross-reactive NAb response and protection against DENV1 (A–D), DENV2 (E–H), and DENV4 (I–L) at the ninth week after the immunization (n = 7). (A,E,I) Endpoint titers of cross-reactive NAbs in sera were detected by PRNT₅₀ and recorded as GMT ± SD. The L.O.D. depicted as a dotted line represents the limit of detection of the assay. (B–D,F–H,J–L). Mice were challenged with DENVs and monitored daily for 27 days. (B,F,J) Pathological symptoms recorded as the mean clinical sign scores. (C,G,K) Percentage of body weight from day 0. (D,H,L) Survival rate shown as the percentage of survivors. Results are representative of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.