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. 2020 Mar 17;11:136. doi: 10.3389/fendo.2020.00136

Figure 5.

Figure 5

hsa-miR-183-5p and miR-21 was a direct target of OTUD6B-AS1. (A) Schematic diagram of screening potential target of OTUD6B-AS1. (B) The sequences of the binding sites of lncRNA OTUD6B-AS1 with miR-21 and miR-183-5p. (C) luciferase reporter assays of SW579 and TPC-1 cells. psiCHECK-2-WT co-transfected with mimics or inhibitor of miR-183-5p or miR-21 in 293T cells. A scramble RNA (NC) was used as control for mimics of miR-183-5p and miR-21. Inhibitor NC was used as control for inhibitor of miR-183-5p and miR-21. Data were presented as mean ± sd (n = 3). ***p < 0.001, NC vs. miR-183-5p or miR-21. Inhibitor NC vs. inhibitor of miR-183-5p or miR-21. (D) The expression of miR-183-5p and miR-21 in normal thyroid cells Nthy-ori, and thyroid carcinoma cells SW579, TPC-1 were examined using qRT-PCR. Data were presented as mean ± sd (n = 3). ***p < 0.001, Nthy-ori vs. SW579 or TPC-1. (E) The ectopic expression of OTUD6B-AS1 caused decreased expression of hsa-miR-183-5p and miR-21 in SW579 and TPC-1 cells. The expression of hsa-miR-183-5p and miR-21 in SW579 and TPC-1 cells were examined using qRT-PCR. U6 was used as internal reference. Data were presented as mean ± sd (n = 3). ***p < 0.001, vector vs. OTUD6B-AS1.