Fig. 3. Adult-onset, MBH-specific ablation of Sh2b1 results in obesity, insulin resistance, and liver steatosis.
AAV1-hSyn-Cre or AAV1-hSyn-GFP vectors were bilaterally injected into the MBH of Sh2b1f/f males at 10 weeks of age. a Growth curves. b Fat content in 10 weeks post transduction. c Energy expenditure in 10 weeks after transduction. d Rectal temperature in 10 weeks following transduction. e Food intake at 10 weeks post transduction. f Overnight fasting plasma insulin levels in 10 weeks after transduction. g GTT and ITT in 10–11 weeks post transduction. h Mice were stimulated with insulin in 11 weeks post transduction. Liver extracts were immunoblotted with the indicated antibodies (n = 3 mice per group). i Representative Oil Red O staining of liver sections and liver TAG levels (normalized to liver weight) in 11 weeks post transduction. a, b, d, e, g, i: AAV1-hSyn-GFP: n = 7, AAV1-hSyn-Cre: n = 7; c: AAV1-hSyn-GFP: n = 7, AAV1-hSyn-Cre: n = 5; f: AAV1-hSyn-GFP: n = 5, AAV1-hSyn-Cre: n = 5. Scale bar: 100 μm. Data are presented as mean ± SEM. *p < 0.05, two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.