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. 2020 Mar 23;10:5230. doi: 10.1038/s41598-020-61678-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summary of representative libraries for each ATAC-seq protocol iteration, produced from cryopreserved chicken lung nuclei. Row colors indicate different iterations of the ATAC-seq protocol. Library names indicate “Protocol iteration–Animal–Nuclei input(–Replicate)”. Progressive changes to the protocol improved signal (Fraction of Reads in Peaks (FRiP) score) and improved overlap with DNase-I Hypersensitive Sites (DHS) and active histone modifications H3K27ac, H3K4me1, and H3K4me3 (minimum 1 bp overlap).