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. 2020 Mar 23;11:1521. doi: 10.1038/s41467-020-15329-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — (a) Graphical abstract of NanoString^TM-nCounter® based in vivo gene expression analysis in a murine model of systemic cryptococcosis through intranasal instillation. Total RNA was extracted from the infected lung, spleen, kidney, and brain tissues recovered after 3, 7, 14, and 21 dpi. Three mice per cohort were used (total 12 mice). Gene-specific nCounter codesets were designed for 58 published virulence-related genes, 183 kinases, 180 TFs, and 8 housekeeping genes for expression normalisation (Supplementary Data 1 and Supplementary Fig. 3). (b-e) Fold-change heatmap of in vivo gene expression of (b) 58 published (known) virulence-related genes, (c) 42 lung-infection-related genes, (d) 22 brain-infection-related genes, and (e) 43 core-virulence genes.