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. 2020 Mar 23;11:1519. doi: 10.1038/s41467-020-15183-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Pulldowns in which FLAG-CHD4 is immobilized on FLAG-Sepharose beads and incubated with the indicated nucleosomes, followed by elution with FLAG peptide. Nucleosome input, unbound nucleosome/flow through (FT), final wash (FW) and elutions of each nucleosome construct were analysed by SDS-PAGE, along with a size indicator, Mark12 Standard (M). The negative control contains no nucleosome in the input. b EMSAs showing the binding of CHD4 to the indicated nucleosomes. Nucleosome concentration was 90 nM (0w0) or 60 nM (0w30 and 0w60). Open and filled triangles indicate complexes that probably contain one or two CHD4 molecules, respectively.