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. 2020 Mar 23;11:1519. doi: 10.1038/s41467-020-15183-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic showing the labelling scheme for 0w(9^AF555)60^Bio and nucleosomal conformations before and after CHD4 remodelling. b FRET vs time traces of 0w(9^AF555)60^Bio alone with a proximal (top) and distal (bottom) AF647-labelled H2A. c FRET vs time trace of 0w(9^AF555)60^Bio bearing a distal AF647 label, showing a gradual increase upon the addition of 2 nM CHD4. This newly established structure can be relatively stable (left) or can be transient, dropping back to the initial state (right). The proportions of each scenario (from 74 molecules) are illustrated as a pie chart. d FRET vs time trace of 0w(9^AF555)60^Bio bearing a distal AF647 label, showing an increase upon the addition of 2 nM CHD4 and 1 mM AMP-PNP. e FRET vs time traces of 0w(9^AF555)60^Bio bearing a distal AF647 label showed a greater increase during CHD4 remodelling in presence of ATP, comparing to the changes induced by CHD4 alone.