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. 2020 Mar 23;11:1519. doi: 10.1038/s41467-020-15183-2

Fig. 6. CHD4 induces similar FRET changes in extra-nucleosomal DNA at the TA-rich side.

Fig. 6

a Schematic showing the labelling scheme for 9AF555w60Bio and the two possible remodelling directions after treating with CHD4, depending on whether the ‘9’ end acts as an entry or an exit side. b FRET vs time traces for 9AF555w60Bio alone with a proximal (top) or distal (bottom) H2A AF647 label. c FRET vs time trace for 9AF555w60Bio bearing a distal AF647 label, showing an increase upon the addition of 2 nM CHD4. This newly established structure can be relatively stable (left) or can be transient, dropping back to the initial state (right). The proportions of each scenario (from 78 particles) are illustrated as a pie chart. d FRET vs time trace for distally labelled 9AF555w60Bio in presence of both 2 nM CHD4 and 1 mM ATP, showing an increase to a similar level to that of CHD4 alone. e FRET vs time trace for proximally labelled 9AF555w60Bio, showing a stepwise drop upon treatment with 2 nM CHD4 and 1 mM ATP. In this case, the AF555 tag at the ‘9’ end is moving away from the histone octamer, in contrast to the direction of movement observed in (d).