FIGURE 4.

Analysis of knockout strains of P. putida KT2440. Biomass growth (A) and 1,4-butanediol concentrations (B) of the wildtype, evolved strains and the ΔpedE-I knockout cultivated in shake flasks in MSM with 20 mM 1,4-butanediol. Growth of selected strains on 20 mM 4-hydroxybutyrate (C). Growth of selected strains on MSM agar plates with 20 mM 1,4-butanediol after 24 h (D) and 48 h (E). Strain numbers next to the plates correspond to full strain names listed in Table 1. The contrast of the images was increased by 20% to improve visibility. Error bars indicate the deviation of the mean (n = 2).