Fig. 5.

Recovery of myeloid and lymphoid cells from newborn and adult mice brains. Tissue from neonatal (A) and adult brain (B) was mechanically dissociated while digested with papain (P), collagenase II (C), dispase II (D) or in the absence of enzymes (NE); and brain homogenates were stained for flow cytometry analysis. Representative plots for each isolation protocol are included. Gates drawn correspond to resting microglia (CD11b+/CD45^low), activated microglia/macrophages (CD11b+/CD45^high), and lymphocytes (CD11b-/CD45+). Cell death within resting microglia was measured after DAPI staining. Associated graphs correspond to % of each population and resting microglia death. Values in all graphs are expressed as percentage of total cells and represent mean ± S.E.M. (n = 4–5). *, p < 0.05; **, p < 0.01; ***, p < 0.001.