Table 1.
Antibodies and reagents used for flow cytometry analysis.
| Antibody/reagent | Clone | Cell targets | Concentration | Company (reference) |
|---|---|---|---|---|
| ACSA-1-PE (anti-GLAST) | ACSA-1 | Neonatal astrocytes and radial glia | 1:25 | Miltenyi Biotec (130-118-483) |
| ACSA-2-APC | IH3-18A3 | Neonatal and adult astrocytes | 1:25 | Miltenyi Biotec (130-117-535) |
| FcR Blocking Reagent | 1:100 | Miltenyi Biotec (130-092-575) | ||
| Calibration Beads | 1000 beads/ml | Miltenyi Biotec (130-093-607) | ||
| CD11b-PE Vio770 | REA592 | Macrophages, microglía, granulocytes, NK cells and subsets of dendritic cells | 1:50 | Miltenyi Biotec (130-113-246) |
| CD206-APC Vio770 | C068C2 | Dendritic cells and subets of macrophages and microglia | 1:10 | BioLegend (141708) |
| DAPI (VioBlue gate) | Dead cells | 1 μg/ml | Sigma-Aldrich (D9542) | |
| CD3-APC | REA641 | T cells | 1:25 | Miltenyi Biotec (130-122-943) |
| CD38-APC | REA616 | Subsets of macrophages, microglía, B cells and T cells | 1:10 | Miltenyi Biotec (130-109-259) |
| CD45-PE | 30F11 | Hematopoietic cells except erythrocytes | 1:25 | Miltenyi Biotec (130-117-498) |