Fig. 2.

In planta Agrobacterium-mediated genetic transformation of horse gram (var. Paiyur 2). a Germinated horse gram seeds used for the infection of Agrobacterium tumefaciens strain. b 7-day-old acclimatized putatively transformed plant. c 14-day-old acclimatized putatively transformed plant. d 50-day-old acclimatized putatively transformed plant by seedlings co-cultivated with Agrobacterium tumefaciens strain EHA105 harboring pCAMBIA2301 binary plasmid. e Putatively transformed plants shows the flowering. f Pod formation at 55 days. g GUS histochemical staining was performed in T₁ horse gram seedlings. h Leaves show GUS expression. i Non-transferred (control) plant leaves show no GUS expression at all