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. 2020 Mar 17;11:436. doi: 10.3389/fmicb.2020.00436

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Copyright © 2020 Lin, Zheng, Bai, Xu, Lin, Chen, Sun, Qu, Yu and Deng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative gene expression of rmpA in 29 clinical isolates of K. pneumoniae. The hvKP and cKP isolates were cultured in LB for 4 h. Total RNA was extracted and transcriptional levels of rmpA were examined by qRT-PCR. The housekeeping gene rrsE was used as the endogenous reference gene. The K. pneumoniae NTUH-K2044 was used as the reference strain (transcriptional level = 1.0). All qRT-PCRs were carried out in triplicate. The hvKP isolates used in this assay were aerobactin positive and hypermucoviscous.