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Detection of serum neutralizing antibodies against A. baumannii phage mixture. Dilutions were made from serum collected from naïve mice given PBS (control) or phage mixture and incubated with a known titer of phage mixture (4 × 104 total PFU). (A) The mixtures were then added to an inoculum of AB5075 and plated in warm agar. (B) After 24 h incubation at 37°C, rate of phage neutralization was measured. N = 7 for PBS, N = 13 for Phage per time point; ***p < 0.001.
