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Investigating intratumor heterogeneity and the TME with single cell approaches. A lung tumor resection is dissociated into single cell suspension which can be used in different applications. CyTOF uses metal-labeled antibodies to detect a limited number of proteins in the cells. Single cell RNA-Seq reveals the transcriptome of each individual cell. Both can be analyzed through computational strategies to dissect intratumor heterogeneity.
