Table 1.
Genes | Forward Primer | Reverse Primer |
P-53 | 5ʹ-TAACAGTTCCTGCATGGGCGGC-3’ | 5ʹAGGACAGGCACAAACACG CACC3’ |
P21 | 5ʹ-ATG TGT GTG GAG AGC GTC AAC C-3’ | 5ʹ-GCA TCC CAG CCT CCG TTA TC-3’ |
Bax | 5ʹ-CCT TTT CTA CTT TGC CAG CAA AC-3’ | 5ʹ-GAG GCC GTC CCA ACC AC-3’ |
BCl2 | 5ʹ-AGGTGACACTATAGAATA-3’ | 5ʹ-GGGATATCACTCAGCATG-3’ |
GAPDH | 5ʹ-GTGCCGGTTCAGGTACTCAGTCA-3’ | 5ʹ-TTGTGGCCTTCTTTGAGTTCGGTG-3’ |
Notes: Adefolaju GA, Theron KE, Hosie MJ. In vitro effects of protease inhibitors on BAX, BCL-2 and apoptosis in two human breast cell lines. S Afr J Sci. 2015; 111(11/12), Art. #2014-0417, 9 pages. http://dx.doi.org/10.17159/sajs.2015/20140417.