Figure 7.

Effects of miR-200a overexpression on cisplatin resistance of LUAD cells. (A) β-Catenin expression in A549-ATB-OE cells transfected with or without miR-200a mimics was detected using RT-qPCR and Western blot assays. (B) Proliferation of A549-ATB-OE cells transfected with or without miR-200a mimics following cisplatin treatment (2 μg/mL) for 48 h was validated by CCK-8 assay. (C) IC₅₀ of cisplatin in A549-ATB-OE cells transfected with or without miR-200a mimics. (D) β-Catenin expression in A549-ATB-OE cells treated with or without Wnt/β-catenin signaling pathway inhibitor IWR-1 (500 nM) for 48 h. (E) Proliferation of A549-ATB-OE cells treated with or without IWR-1 (500 nM) for 48 h following cisplatin treatment (2 μg/mL) for 48 h was validated by CCK-8 assay. (F) IC₅₀ of cisplatin in A549-ATB-OE cells treated with or without IWR-1 (500 nM) for 48 h. Data are expressed as mean ± SD (n=3). *P<0.05; NS, not significant.