FIGURE 3.

Carbon partitioning between the photoautotrophic shoot and heterotrophic root of 10-days old wheat seedlings. (A) Long-distance transport from the shoot to root in AVP1 Null, AVP1-1 and AVP1-2 plants using ¹⁴CO2 over light and dark periods. n = 3 plants per time point, per experiment, results from two independent experiments; error bars represent standard error of the mean. DPM, Disintegrations per minute. (B) Root growth of transgenic wheat plants expressing AVP1 under optimal growth conditions at 6 weeks after sowing. (C) Root biomass at 3 weeks after sowing. (D) Primary roots total length at 3 weeks after sowing. Plants from two independent AVP1 transformation events were grown in an inorganic kiln-fired porus ceramic matrix for three weeks prior to their root system being imaged using an Epson flatbed scanner before root mass was dried at 80°C oven for 4 days and the weight recorded. Results are the mean ± SE (n = 5 independent plants). Significant differences between transgenic and null plants using Dunnett’s multiple comparison test: *P < 0.05.