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. 2020 Feb 28;20:217–230. doi: 10.1016/j.omtn.2020.02.010

Figure 2.

Figure 2

Rescue of ube3d Morphants

(A–C) (A) Live images of 24 hpf WT; (B) Live images of 24 hpfMO; (C) Live images of rescue 24-hpf larvae. (D–I) (D and G) Live images of 96 hpf WT; (E and H) Live images of 96 hpf MO; (F and I) Live images of Rescue 96-hpf larvae. (G) Enlargement of (D), (H) Enlargement of (E), (I) Enlargement of (F). (J) At 96 hpf, the ube3d MO embryos were partially rescued by coinjection with human ube3d mRNA, and the percentage of small eyes in the rescued larvae was significantly lower than the percentage in MO larvae.