Fig. 1.

Phylogenetic tree analysis of W02B12.15. (A) Amino acid sequence alignment of CISD proteins among C. elegans, D. rerio, D. melanogaster, M. musculus and H. sapiens. The single letters denote amino acids, of which highly conserved residues are colored. Numbers indicate the position of amino acids. (B) Schematic view of the transmembrane and CDGSH domains of CDGSH-containing proteins and their conservation among C. elegans, D. rerio, D. melanogaster, M. musculus and H. sapiens. The domain architectures were analyzed by SMART (a Simple Modular Architecture Research Tool). (C) C. elegans W02B12.15 gene is evolutionarily close to the Cisd-1 clades. Neighbor joining phylogenetic tree analysis of the CDGSH domain-containing proteins from different model organisms were calculated based on identity (%).