Fig. 2.

Characterization of protein encoded by W02B12.15. (A) Genomic structure of W02B12.15 and two possible transcripts encoded by the gene. The deletion allele in the mutant cisd-1(tm4993) is indicated, from nucleotide positions 424 to 745. (B) Scheme of the construct Pfib-1::cisd-1::gfp::icr::linker::3′UTRunc-54-AMPR used for generation of transgenic worms (cguIs14) (strain SJL40). (C) Western blot analysis of four strains of worms - wild-type (N2), cisd-1(tm4993), SJL40 (transgenic worm), and SJL41 (transgenic worm in tm4993 background) - using anti-GFP (left) and anti-mouse CISD-1 (right) antibodies.