Fig. 1. Expression levels of signals via the succinate receptor 1 (SUCNR1) mRNA in various tissues of mice. A 12-week-old C57BL/6J male mouse was euthanized before the organs were isolated and snap frozen with liquid nitrogen. The samples were stored at −80℃ until RNA extraction. An age matched C57BL/6J female mouse's ovary was collected and stored in the same way. Total RNA was isolated using TRI Reagent (Sigma-Aldrich), and purified using the RNeasy kit (Qiagen). Following TaqMan reverse transcription, polymerase chain reaction was performed in a BioRad 384-module using a SYBR Green (Applied Biosystems) method with primers for the indicated genes: β-actin forward primer: 5′-AGCCATGTACGTAGCCATCC-3′; β-actin reverse primer:5′-CTCTCAGCTGTGGTGGTGAA-3′. SUCNR1 forward primer: 5′-GACAGAAGCCGACAGCAGAAT-3′; SUCNR1 reverse primer: 5′-TAGACATTGCTGCTGTTCCAGTT-3′.
