Table 3.
Targets selected for genome engineering of P. putida.
| Gene(s) number | Gene(s) name | Genomic region [bp (strand)] | Length (bp) | Annotated functiona |
|---|---|---|---|---|
| PP_1239 | 1,416,753–1,417,511 (+) | 759 | MBLFAb | |
| PP_3291 | 3,725,348–3,726,715 (+) | 1,368 | MBLFA | |
| PP_1952 | 2,208,685–2,209,632 (+) | 948 | MBLFA | |
| PP_0772 | 890,074–890,718 (+) | 645 | MBLFA | |
| PP_2876 | ampC | 3,276,978–3,278,120 (—) | 1,143 | β-lactamase |
| PP_1775 | 1,982,049–1,983,482 (+) | 1,434 | MBLFA | |
| PP_0052 | 60,831–61,715 (+) | 885 | β-lactamase domain-containing protein | |
| PP_2045 | 2,325,342–2,327,303 (—) | 1,962 | MBLFA | |
| PP_3161-PP_3164 | benABCD | 3,581,930–3,585,749 (+) | 3,819 | Benzoate catabolism gene clusterc |
| PP_4219 | pvdD | 4,768,854–4,779,266 (—) | 10,412 | Non-ribosomal peptide synthetase |
a
Functional annotations and genome coordinates are given according to the Pseudomonas Database (Winsor et al., 2016) and Belda et al. (2016).
b
MBFA, metallo-β-lactamase family protein.
c
The enzymes encoded in the ben gene cluster are BenA, subunit α of benzoate 1,2-dioxygenase; BenB, subunit β of benzoate 1,2-dioxygenase; BenC, electron transfer component of benzoate 1,2-dioxygenase; and BenD, 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase.