REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Tim22	This paper	#164
Rabbit polyclonal anti-Tim54	This paper	#215
Rabbit polyclonal anti-Tim18	This paper	#233
Rabbit polyclonal anti-Tim23	This paper	#3846
Rabbit polyclonal anti-Tim17	This paper	#4968
Rabbit polyclonal anti-Tim50	This paper	#3314
Rabbit polyclonal anti-Tim21	This paper	#3111
Rabbit polyclonal anti-Tim44	This paper	#3869
Rabbit polyclonal anti-Tom40	This paper	#4901
Rabbit polyclonal anti-Hsp70	This paper	#4945
Rabbit polyclonal anti-Mir1	This paper	#171
Rabbit polyclonal anti-Aac2	This paper	#51
Rabbit polyclonal anti-Yhm2	This paper	#3053 (Freiburg)
Rabbit polyclonal anti-Mpc1	This paper	#5021 (Freiburg)
Rabbit polyclonal anti-Atp20	This paper	#1517
Rabbit polyclonal anti-Mic10	This paper	#345
Rabbit polyclonal anti-Atp5	This paper	#1546
Rabbit polyclonal anti-MPC1	Thermo Fisher Scientific	Cat# PA5-60929; RRID: AB_2638597
Rabbit polyclonal anti-MPC2	Proteintech	Cat# 20049-1-AP
Rabbit polyclonal anti-TIM22	Proteintech	Cat# 14927-1-AP; RRID: AB_11183050
Rabbit polyclonal anti-AGK	This paper	#5045
Rabbit polyclonal anti-ANT3	Proteintech	Cat# 14841-1-AP; RRID: AB_2190371
Rabbit polyclonal anti-TIM23	This paper	#1526
Rabbit polyclonal anti-LETM1	This paper	#0538
Rabbit polyclonal anti-COX1	This paper	#5120
Rabbit polyclonal anti-COX4-1	This paper	#1522
Rabbit polyclonal anti-ATP5B	This paper	#4826
Goat anti Rabbit IgG (H+L) HRPO	Jackson ImmunoResearch Labs	Cat# 111-035-144; RRID: AB_2307391
Chemicals, Peptides, and Recombinant Proteins
Digitonin	Merck Millipore	Cat# 300410
MitoTrackerTM Orange CMTMRos	Thermo Fisher Scientific	Cat# M-7510
3,3′-Dipropylthiadicarbocyanine Iodide (DiSC3)	Invitrogen	Cat# D306
deuterated cyanoborohyride NaBD3CN	Sigma-Aldrich	Cat# 190020
deuterated formaldehyde CD2O	Sigma-Aldrich	Cat# 492620
deuterated 13C-labeled formaldehyde 13CD2O	Sigma-Aldrich	Cat# 596388
[35S] methionine	Hartmann Analytic	Cat# SCM-01
Critical Commercial Assays
KOD Hot Start DNA Polymerase	Merck	Cat# 71086-3
mMessagemMachine SP6 transcription kit	Invitrogen	Cat# AM1340
Flexi Rabbit Reticulocyte Lysate System	Promega	Cat# L4540
Experimental Models: Cell Lines
HEK293-Flp-InTM T-RexTM (HEK293T) Cell Line	ThermoFisher Scientific	RRID: CVCL_U421
Control Fibroblasts (immortalized)	[33]	N/A
TIM22 Patient Fibroblasts (immortalized)	[33]	N/A
Experimental Models: Organisms/Strains
YPH499 MATa ade2-101, his3-Δ200, leu2-Δ1, ura3-52, trp1-Δ63, lys2-801	[36]	Yeast collection# 13
BY4741 MATa his3Δ1, leu2Δ0, met15Δ0, ura3Δ0	EUROSCARF	http://euroscarf.de/index.php?name=News
BY4741 yfr045wΔ::KanMX4	EUROSCARF	http://euroscarf.de/index.php?name=News
BY4741 ypr011cΔ::KanMX4	EUROSCARF	http://euroscarf.de/index.php?name=News
BY4741 mpc1Δ::KanMX4	EUROSCARF	http://euroscarf.de/index.php?name=News
tim22-14: YPH499 tim22-M4	[19]	Yeast collection# 172
tim10-2: YPH499 tim10::ADE2	[10]	Yeast collection# 173
YPH499 mpc1Δ: YPH499 mpc1Δ::KanMX4	This paper	N/A
tim22-14/mpc1Δ: YPH499 tim22-M4 mpc1Δ::KanMX4	This paper	N/A
tim10-2/mpc1Δ: YPH499 tim10::ADE2 mpc1Δ::KanMX4	This paper	N/A
tom70/71 ΔΔ: YPH499 tom70Δ::HIS3, tom71Δ::KanMX4	[20]	Yeast collection# 796
Tim22↓: YPH499 KanMX-pGal1-Tim22	This paper	N/A
Oligonucleotides
TIM22 siRNA GUG-AGG-AGC-AGA-AGA-UGA	[37]	N/A
Recombinant DNA
Plasmid: GFP-Mir1	This paper	pRG3
pFA6a-KanMX6	[38]	Plasmid# R66
Software and Algorithms
ImageQuantTL v8.1	GE Healthcare	https://www.gelifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110
ImageJ v1.47	NIH	https://imagej.nih.gov/ij/download.html
Geneious v9.1.2	Biomatters Ltd	https://www.geneious.com/download/
Prism 8	GraphPad Software	https://www.graphpad.com/scientific-software/prism/