Figure 6.

Gene Expression Profile of ADC Arising from SPC^POS and CGRP^POS Reveals Differences Associated with the Cell of Origin

(A) PCA analysis on triplicates of SCLC, ADC developed by Ad5-CGRP-Cre-injected RP-Fgfr1 mice (SCLC-CGRP; ADC-CGRP), and ADC developed by Ad5-SPC-Cre-injected RP-Fgfr1 mice (ADC-SPC), defined by variables PC1 and PC2.

(B) Heatmap of significantly (FDR <0.05) upregulated and downregulated genes between SCLC and ADC originating from CGRP^POS cells (C-ADC) versus ADC initiated from SPC^POS cells (S-ADC). Gene ontology and functional analysis performed with DAVID program identified the indicated terms (legend with different colors).

(C–N) FGFR1 (C–E), SOX2 (F–H), and SOX9 (I–K) staining on SCLC (CGRP-SCLC; C, F, I, and L) and ADC (CGRP-ADC; D, G, J, and M) developed by Ad5-CGRP-Cre-injected RP-Fgfr1 mice, and ADC developed by Ad5-SPC-Cre-injected RP-Fgfr1 mice (SPC-ADC; E, H, K, and N). TTF1 staining is used as positive control of ADC (L–N). Scale bar indicates 50 μm.

See also Figure S6.