Table 1.
Percent recoveries of transmissible gastroenteritis virus (TGEV) and hepatitis A virus (HAV) seeded in 5-L surface water samples employing adsorption onto glass wool and different elution procedures
| Elution | Virus | |||
|---|---|---|---|---|
| TGEV | HAV | |||
| Adsorption | Elution | Adsorption | Elution | |
| pH 9.5; 10 mina | 71.6 | 2.6 | 59.6 | 7.2 |
| pH 11.0; 10 min | 51.4 | 28.8 | 50.2 | 33.1 |
| pH 11.0; O/N | 62.9 | 37.4 | 52.8 | 34.3 |
| pH 11.0 + Tween 80 0.3%; O/N | 42.7 | 100.0 | 45.6 | 86.7 |
| Average adsorption (mean ± SE) | 57.1 ± 6.3 | – | 52.1 ± 2.9 | – |
The composition of the elution buffer was 0.05 M glycine with 3% of beef extract
O/N overnight, SE standard error
aStandard elution procedure