Table 2.
Percent recoveries of transmissible gastroenteritis virus (TGEV) and hepatitis A virus (HAV) seeded in 50-L surface water samples employing adsorption onto glass wool and different elution procedures
| Elution | Virus | |||
|---|---|---|---|---|
| TGEV | HAV | |||
| Adsorption | Elution | Adsorption | Elution | |
| pH 11.0; O/N | 73.8 | 2.9 | 44.1 | 6.8 |
| pH 11.0 + Tween 80; O/N | 64.3 | 0.4 | 41.9 | 22.6 |
| pH 11.0; agitation, O/N | 50.1 | 10.4 | 23.2 | 23.3 |
| pH 11.0; recirculation 20 min | 59.2 | 18.0 | 27.1 | 23.9 |
| Average adsorption (mean ± SE) | 61.9 ± 9.9 | 34.1 ± 10.5 | ||
The composition of the elution buffer was 0.05 M glycine with 3% of beef extract
O/N overnight, SE standard error