Fig. 3.

Qualitative evaluation of different siRNAs expressing BHK-21 cell lines to target RV-L and RV-N gene transcripts. Different homogenous siRNAs expressing BHK-21 cell lines (BHK-L or BHK-N) were transfected with target gene expressing plasmids (pDsRed-L or pDsRed-N) expressing RV-L or N genes with C-terminal fusion with red fluorescent protein (RFP). At 48-h post-transfection, cells were analyzed for reduction in L-RFP (a) and expression N-RFP (b) compared with transfected BHK-21 control cells