Fig. 2.

Detection of RNA hybridization and optimization of experimental conditions. (a) Detection of RNA hybridization. RNA was captured on sensor chip surface, then oligo DNA CR that is complementary to part of the RNA was injected to check the actual response of RNA hybridization. (i) Biotinylated DNA covered chip was stabilized with buffer. (ii) RNA sample was hybridized to biotinylated DNA. (iii) RNA was captured and the chip was stabilized with buffer. (iv) Oligo DNA CR complementary to part of the RNA was injected, giving rise to an additional response units increasing. (v) Non-complementary DNA as negative control was injected and no significant change was detected on the sensorgram. (b) Effect of temperature on RNA hybridization. (c) Effect of temperature on oligo DNA CB hybridization