Table 1.
High-confidence proteins identified in purified RABV virions by LC–MS/MS.
| Protein ID | Protein name | Description | Unique peptidesa | Sequence coverage (%)b | Abundancec | Mass (kDa) |
Subcellular locationd | Reported in other virusese |
|---|---|---|---|---|---|---|---|---|
| O92284 | G | Glycoprotein | 37 | 49 | 1.75E+10 | 58.86 | Virion | |
| Q8JXF6 | N | Nucleoprotein | 40 | 80.7 | 1.48E+10 | 50.73 | Virion | |
| P22363 | P | Phosphoprotein | 32 | 84.2 | 1.13E+10 | 33.62 | Virion | |
| P25223 | M | Matrix protein | 8 | 52.5 | 7.75E+09 | 23.13 | Virion | |
| D8VEC2 | L | Large structural protein | 111 | 61.5 | 3.94E+09 | 242 | Virion | |
| P40240 | CD9 | CD9 antigen | 7 | 26.5 | 1.72E+09 | 25.26 | Membrane | IAV1, HAV, MEV, HIV1, ASFV |
| P63168 | DLC8 | Dynein light chain 1 | 2 | 57.3 | 1.09E+09 | 10.37 | Nucleus, mitochondria, cytoskeleton | |
| P63017 | HSC70 | Heat shock cognate 71 kDa protein | 28 | 55.9 | 5.83E+08 | 70.87 | Membrane, nucleus, cytoplasm | RSV, HIV1, HIV2,VSV, RVFV, HSV, ASFV, JUNV |
| P17742 | CyPA | Cyclophilin A | 9 | 68.3 | 5.65E+08 | 17.97 | Cytoplasm | IAV1, MEV, HIV1, HIV2, HIV3, HSV, KSHV |
| P63001 | RAC1 | RAS-related C3 botulinum | 6 | 36.5 | 3.51E+08 | 23.43 | Membrane, cytoplasm | RVFV, HIV2 |
| P18760 | Cofilin-1 | Cofilin-1 | 14 | 58.1 | 3.27E+08 | 24.58 | Cytoskeleton | IAV1, RSV, HIV1, HIV2, HSV |
| P35762 | CD81 | CD81 antigen C | 4 | 30.9 | 2.99E+08 | 25.81 | Membrane | IAV1, HCV, MEV, HIV1, HIV2,VV |
| P61205 | ARF3 | ADP-ribosylation factor 3 | 4 | 57.5 | 2.93E+08 | 20.60 | Golgi | HSV |
| P0CG50 | Ubc | Polyubiquitin-C | 7 | 73.6 | 2.41E+08 | 82.55 | Nucleus, cytoplasm | IAV2, RSV, HIV1, VSV, JUNV |
| O08992 | Syntenin-1 | Syntenin-1 | 8 | 40.5 | 1.77E+08 | 32.38 | Membrane, cytoplasm, cytoskeleton, nucleus, ER, junction | HIV2 |
| Q9WVE8 | PACSIN 2 | Protein kinase C and casein kinase substrate in neurons protein 2 | 13 | 27 | 1.45E+08 | 55.83 | Cytosol, endosome, nucleus, caveola | |
| P10852 | Slc3a2 | 4F2 cell-surface antigen heavy chain | 19 | 39.7 | 1.03E+08 | 58.34 | Membrane | RSV, HIV1, VSV |
| O35566 | CD151 | CD151 antigen | 5 | 17 | 8.78E+07 | 28.25 | Membrane | RVFV |
| P41731 | CD63 | CD63 antigen | 3 | 10 | 8.72E+07 | 26.78 | Membrane | HCV |
| Q9R0P5 | Destrin | Destrin | 6 | 41.2 | 8.62E+07 | 18.52 | Cytoskeleton | IAV1 |
| P60766 | CDC42 | Cell division control protein 42 homolog | 6 | 42.4 | 8.24E+07 | 21.26 | Membrane, cytoskeleton, cytoplasm | IAV2, HIV1, SARS |
| Q9D8B3 | CHMP4B | Charged multivesicular body protein 4b | 7 | 41.1 | 6.07E+07 | 24.94 | Late endosome, cytosol | HAV |
| P63037 | HSP40 | DnaJ homolog subfamily A member 1 | 11 | 36.5 | 5.91E+07 | 44.87 | Membrane, nucleus, ER, mitochondria, cytoplasm | HIV2 |
| P16858 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 9 | 30.6 | 5.73E+07 | 38.65 | Cytoskeleton, cytosol, nucleus | IAV1, RSV, HIV1, HIV2, HIV3, RVFV, IBV, ASFV, KSHV |
| P63242 | EIF5a | Eukaryotic translation initiation factor 5A-1 | 6 | 45.5 | 5.71E+07 | 16.83 | Nucleus, ER | KSHV |
| Q4VAE6 | RhoA | Ras family member A | 5 | 28.5 | 5.67E+07 | 21.80 | Nucleus, ER, cytoplasm | IBV |
| O54946 | HSJ-2 | DnaJ homolog subfamily B member 6 | 6 | 21.9 | 5.16E+07 | 39.81 | Nucleus | |
| P63024 | VAMP3 | Vesicle-associated membrane protein 3 | 2 | 32 | 4.84E+07 | 11.48 | Membrane | |
| Q9WU78 | ALIX | Programmed cell death 6-interacting protein | 30 | 38.9 | 4.84E+07 | 96.31 | Cytoskeleton, cytosol | HAV, HIV1, VSV, HSV, JUNV |
| P63101 | 14–3-3 ζ/θ | 14–3-3 protein zeta/delta | 6 | 37.6 | 4.66E+07 | 27.77 | Cytoskeleton | HIV1, HSV, KSHV, SARS |
| Q62167 | DDX3X | ATP-dependent RNA helicase DDX3X | 5 | 41.2 | 4.13E+07 | 73.10 | Mitochondria, nucleus | HSV, JUNV, SARS |
| P99024 | Tubulin β-5 | Tubulin beta-5 chain | 4 | 35.8 | 4.05E+07 | 49.67 | Cytoplasm, cytoskeleton, microtubules | IAV1, RSV, HIV1, VSV, ASFV |
| P11499 | HSP90β | Heat shock protein HSP 90-beta | 15 | 34.4 | 3.94E+07 | 83.28 | Membrane, cytoplasm, nucleus | RSV, HAV, HIV1, VSV, RVFV, IBV, KSHV, SARS |
| Q91ZR2 | SNX | Sorting nexin-18 | 10 | 20 | 3.76E+07 | 67.79 | Membrane | |
| Q61187 | TSG101 | Tumor susceptibility gene 101 protein | 7 | 19.2 | 3.40E+07 | 44.12 | Endosome, nucleus | HIV1, VSV, JUNV |
| P17182 | ENO1 | Alpha-enolase | 9 | 24 | 3.17E+07 | 47.14 | Membrane, cytoplasm | IAV1, HAV, MEV, HIV1, HIV2, VSV, IBV, ASFV, KSHV |
| Q9DB34 | CHMP2A | Charged multivesicular body protein 2a | 5 | 18 | 3.09E+07 | 25.13 | Cytoplasm, late endosomes | |
| Q9Z127 | SLC7 | Large neutral amino acids transporter small subunit 1 | 5 | 8.8 | 2.94E+07 | 55.87 | Membrane, cytosol | |
| P35278 | Rab5C | Ras-related protein Rab-5C | 4 | 19.7 | 2.91E+07 | 25.35 | Membrane, endosomes | HAV, HIV1, HIV2, ASFV, HSV |
| Q3UFR4 | SLC1 | Amino acid transporter | 8 | 18.9 | 2.60E+07 | 58.36 | Membrane | |
| Q99J93 | IFITM | Interferon-induced transmembrane protein 2 | 2 | 23.6 | 2.30E+07 | 15.74 | Membrane | |
| Q9D1C8 | VPS28 | Vacuolar protein sorting-associated protein 28 homolog | 7 | 36.7 | 2.19E+07 | 25.45 | Endosomes | HIV2 |
| P51150 | RAB7A | Ras-related protein Rab-7a | 6 | 32.9 | 1.88E+07 | 23.49 | Late endosomes | HAV, HIV1, RVFV, HSV, KSHV, JUNV |
| P06837 | GAP43 | Neuromodulin | 7 | 49.8 | 1.79E+07 | 23.63 | Membrane, synapses | |
| P26040 | Ezrin | Ezrin | 8 | 18.3 | 1.77E+07 | 69.41 | Cytoskeleton, cytosol | IAV2, HIV1 |
| P60335 | PCBP1 | Poly(rC)-binding protein 1 | 4 | 18.5 | 1.64E+07 | 37.5 | Nucleus, cytoplasm | HIV1, HIV2, SARS |
| P16045 | Galectin-1 | Galectin-1 | 3 | 28.1 | 1.61E+07 | 14.87 | Cell surface, extracellular matrix | HIV2 |
| P62331 | ARF6 | ADP-ribosylation factor 6 | 3 | 21.1 | 1.11E+07 | 20.08 | Cytoplasm, cytosol, early endosomes | |
| Q91YD9 | nWASP | Neural Wiskott-Aldrich syndrome protein | 5 | 12.6 | 9.01E+06 | 54.27 | Cytoplasm, cytoskeleton, nucleus | |
| P61089 | Ube2 | Ubiquitin-conjugating enzyme E2 N | 5 | 33.6 | 6.31E+06 | 17.14 | Cytoplasm, nucleus | |
| B2RRX1 | β-actin | Beta-actin | 3 | 54.1 | 4.96E+06 | 41.74 | Membrane, cytoskeleton, cytosol | IAV1, HIV1, HIV2, VSV, RVFV, IBV, HSV, KSHV |
| Q8R0J7 | VPS37B | Vacuolar protein sorting-associated protein 37B | 4 | 17.9 | 4.30E+06 | 31.06 | Late endosomes, cytoplasm | |
| P46467 | VPS4B | Vacuolar protein sorting-associated protein 4B | 7 | 18.2 | 3.62E+06 | 49.42 | Late endosomes | HAV |
| P68040 | RACK1 | Receptor of activated protein C kinase 1 | 4 | 11.7 | 2.58E+06 | 35.08 | Membrane, cytoplasm, nucleus |
aThe number of unique peptides correspond to the maximal values among the three biological replications.
bThe percentages of sequence coverage based on peptides with unique sequences.
cAverage abundance expressed by iBAQ calculated from three separate determinations.
dSubcellular location was investigated using the Uniprot database. Endoplasmic reticulum, ER.
eVirus names: influenza A virus (Shaw et al.2008 for IAV1, Mindaye et al. 2017 for IAV2); HAV: hepatitis A virus (McKnight et al.2017); MEV: measles virus (Sviben et al.2018); HIV: human immunodeficiency virus (Linde et al.2013 for HIV1, Chertova et al. 2006 for HIV2, Saphire et al. 2006 for HIV3); ASFV: African swine fever virus (Alejo et al.2018); RSV: respiratory syncytial virus (Radhakrishnan et al.2010)); VSV: vesicular stomatitis virus (Moerdyk-Schauwecker et al.2009); RVFV: rift Valley fever virus (Nuss et al.2014); HSV: herpes simplex virus type 1 (Stegen et al.2013); KSHV: Kaposi’s sarcoma-associated herpesvirus (Zhu et al.2005); HCV: hepatitis C virus (Lussignol et al.2016); VV: vaccinia virus (Krauss et al.2002); IBV: infection bronchitis virus (Kong et al.2010); JUNV: Junin virus (Ziegler et al.2018); SARS: severe acute respiratory syndrome (Neuman et al.2008).