Fig. 1.

2D [¹⁵N,¹H]-HSQC spectrum of uniformly ¹³C,¹⁵N-labeled nsp3(513–651) (protein concentration 1.2 mM, 25 mM sodium phosphate buffer at pH 6.5, 150 mM NaCl, 2 mM NaN₃). The spectrum was recorded at 600 MHz ¹H frequency at a temperature of 25°C, with 256 increments in the ¹⁵N dimension and 4 scans/increment. The side chain amide resonances of asparagine and glutamine are connected by horizontal lines. The backbone resonances are identified by continuous numbering in the construct used, where the numbers 5–143 identify the residues 513–651 of nsp3 (see text)