Fig. 6.

Intracellular retention of Cx43 in endoplasmic reticulum of MHV-A59-infected primary meningeal fibroblast cultures (a–f). Meningeal fibroblasts were mock-infected (a–c) or infected with MHV-A59 (d–f), and double-immunostained for calnexin (green: b, e) and Cx43 (red: a, d). In infected cultures (d–f), Cx43 puncta are mostly seen in the perinuclear region which colocalize with ER marker calnexin (f; arrows). In control cells (a–c), most of the Cx43 staining is observed towards the periphery (c; arrowheads) with only a few Cx43 puncta seen to colocalize with calnexin (c; arrow). Cells were counterstained with DAPI (blue) to visualize nuclear localization in merged images