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NIH3T3/L-SIGN and NIH3T3 were pretreated with HCV E2 prior to HCVcc infection. Total (T-) and phosphorylated (P-) Raf, MEK, and ERK were analyzed by Western blotting. β-actin is shown as a loading control. Changes in the levels of phosphorylated Raf, MEK, and ERK after being normalized to the levels of β-actin are shown below each blot. One representative experiment out of three is shown
