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. 2020 Mar 15;459(2):126–137. doi: 10.1016/j.ydbio.2019.12.009

Fig. 1.

Fig. 1

Dynein is required to traffic Crumbs protein apically in the Drosophila follicle cell epithelium

A) Endogenously tagged knockin Crb-GFP localises apically in the follicle cell epithelium (stage 7/8 egg chamber; scale bar approximately 10 ​μm).

B) Expression of Dynein hairpin RNAi throughout the follicle cell epithelium with tj.Gal4 causes relocalisation of Crb-GFP to the basal surface of follicle cells. Anti-GFP antibody staining is shown (stage 7/8 egg chamber; scale bar approximately 10 ​μm).

C) Clonal expression of Dynein hairpin RNAi, marked by expression of nls-GFP, which causes cell-autonomous relocalisation of Crb to the basal surface of follicle cells within the clone (stage 7/8 egg chamber; scale bar approximately 10 ​μm).

D) Co-immunostaining for Crb and Rab11 upon clonal expression of Dynein hairpin RNAi, marked by expression of nls-GFP, reveals co-localization within basal endosomes (stage 7/8 egg chamber; scale bar approximately 10 ​μm). Asterisks indicate a clone of cells expressing UAS.Dynein-RNAi.