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. 2020 Mar 15;459(2):126–137. doi: 10.1016/j.ydbio.2019.12.009

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Dynein loss is partially rescued by Kinesin-RNAi.

A) Endogenously tagged knockin Crb-GFP localises apically in the follicle cell epithelium (stage 7 egg chamber; scale bar approximately 10 μm).

B) Expression of Dynein hairpin RNAi throughout the follicle cell epithelium with tj.Gal4 causes relocalisation of Crb-GFP to the basal surface of follicle cells (stage 7 egg chamber; scale bar approximately 10 μm).

C) Expression of Kinesin hairpin RNAi throughout the follicle cell epithelium with tj.Gal4 does not affect localization of Crb-GFP in follicle cells (stage 7 egg chamber; scale bar approximately 10 μm).

D) Expression of both Dynein and Kinesin hairpin RNAi throughout the follicle cell epithelium with tj.Gal4 does partially rescues the effect of Dynein RNAi on localization of Crb-GFP in follicle cells. Anti-GFP antibody staining is shown (stage 7 egg chamber; scale bar approximately 10 μm).