Fig. 3.

Dominant-negative Myosin V prevents apical delivery of Crumbs, revealing apical transport of entire Crumbs-containing Rab11-positive endosomes by Dynein.

A) GFP-tagged MyoV expressed with tj.Gal4 localises primarily to the apical domain of follicle cells (n ​> ​10 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

B) GFP-tagged MyoV GT (dominant-negative) localises to endosomes (n ​> ​10 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

C) Control egg chambers immunostained for Dlg and Crb (n ​> ​12 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

D) Expression of dominant-negative MyoV GT prevents apical localization of Crb, which instead accumulates in endosomes that localise towards the apical pole of the cell (n ​> ​15 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

E) High magnification view of C (n ​> ​15 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

F) Co-expression of Dynein hairpin RNAi with dominant-negative MyoV GT causes accumulation of Crb in endosomes that localise basally (n ​> ​11 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

G) Co-immunostaining for Rab11 and Crb upon expression of dominant-negative MyoV GT reveals co-localization in enlarged apical endosomes, which are also positive for MyoV-GT-GFP (see Fig. S1B) (n ​> ​8 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

H) Co-immunostaining for Rab11 and Crb upon expression of dominant-negative MyoV GT and dynein-RNAi hairpins reveals co-localization in enlarged basal endosomes (n ​> ​9 stage 7/8 egg chambers; scale bar approximately 10 ​μm).