Fig. 4.

The F-actin cytoskeleton, which concentrates apically, is required for apical localization of both MyoV and Crb.

A) Control (DMSO-treated) egg chamber stained for F-actin with phalloidin (n ​> ​9 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

B) Latrunculin A treated egg chamber stained for F-actin with phalloidin (n ​> ​10 stage 7/8 egg chambers; scale bar approximately 10 ​μm).

C) Control (DMSO-treated) egg chamber expressing MyoV-GFP with tj.Gal4 (n ​> ​10 stage 7/8 egg chambers; scale bar approximately 10 ​μm). C′ show zoom image.

D) Latrunculin A treated egg chamber expressing MyoV-GFP with tj.Gal4 shows loss of MyoV-GFP apical localization (n ​> ​12 stage 7/8 egg chambers; scale bar approximately 10 ​μm). D′ shows zoom image.

E) Control (DMSO-treated) Crb-GFP egg chamber showing normal apical localiz

ation of Crb in follicle cells (n ​> ​7 stage 7/8 egg chambers; scale bar approximately 10 ​μm). E′ shows zoom image.

F) Latrunculin A treated Crb-GFP egg chamber showing loss of apical Crb localiz

ation and localization to endosomal punctae (n ​> ​5 stage 7/8 egg chambers; scale bar approximately 10 ​μm). F′ shows zoom image.

G) Cytochalasin D treated Crb-GFP egg chamber showing loss of apical Crb localiz

ation and localization to endosomal punctae (n ​> ​7 stage 7/8 egg chambers; scale bar approximately 10 ​μm). G′ shows zoom image.