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. 2020 Mar 23;30(6):972–987.e12. doi: 10.1016/j.cub.2020.01.036

Figure 4.

Figure 4

Membrane Tubulation by Membrane-Attached Kinesin-1

(A) Shown on the left is a representative image of a tubular network observed in the presence of 30 nM kinesin-1-GFP. Shown on the right is a time-lapse images of a membrane tube (red arrowheads) pulled by kinesin-1-GFP (black arrowheads) along a MT (dashed yellow line) in the same conditions.

(B) Kymograph of a membrane tube sliding along a MT and catching up with the MT tip (white arrowhead) in the presence of 15 nM kinesin-1-GFP, 200 nM EB3, and 15 nM His-GFP-MTLS.

(C) Speed of membrane sliding along MTs at 7 nM (n = 35), 15 nM (n = 55), and 30 nM (n = 59) kinesin-1-GFP in the presence of 200 nM EB3 and 15 nM His-GFP-MTLS. The dashed lines represent the mean values of the membrane sliding speed without kinesin (see Figure S4B).

(D) Kymograph of a membrane tube moving together with the plus end of a growing MT in the presence of 15 nM kinesin-1-GFP and 200 nM EB3.

(E) Probability of the indicated successful membrane tube generation events, determined as in Figure 3H. Numbers of events in 2 experiments, from left to right: 15, 61, 8, 26, 45, 38, 36, 31, 21, and 55.